Background β-thalassemia major is a genetic issue depicted by a diminished speed of hemoglobin creation, provoking insufficient association of no less than one globin chain. The reality of shortcomings could change depending upon the specific sickness. The model for this study involved not being set in stone to have β-thalassemia, while an additional fifty individuals without the condition were picked as the benchmark bunch. The Ibn Albaladi Thalassemia Center assembled blood tests During the period from 1^st June to 31 October 31, 2022. The place of this study was to assess the levels of serum immunological markers, expressly IL-6 and IL-8, in patients from Iraq who are assailed with β-thalassemia.The survey plan for postgraduate assessments at the School of Baghdad has been embraced by the Panel of the Association of Innate Planning and Biotechnology. The protein-associated immunosorbent look (ELISA) was utilized to assess the unions of human interleukin-6 (IL-6) and interleukin-8 (IL-8). The discoveries of the review showed that people determined to have β-thalassemia displayed strikingly raised serum groupings of IL-6 and IL-8, with factual importance. It was found that individuals with β-thalassemia displayed IL-6 focuses estimating 30.90±3.39 pg/ml, though the benchmark group exhibited degrees of 20.21±2.88 pg/ml. Likewise, it was seen that the IL-8 level in the benchmark group was estimated to be 74.86±21.01 pg/ml, though the patient gathering showed a higher IL-8 degree of 219.10±47.26 pg/ml. The discoveries of this study show that people with thalassemia display an insusceptible dysregulation portrayed by simultaneous immunosuppression and irritation