Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by dysregulated innate and adaptive immune responses. Eighty cases of SLE involving 40 patients with lupus nephritis with a mean age (33.80±11.824) years (13 males and 27 females) and 40 patients with lupus arthritis with a mean age (34.43±11.942) year (10 males and 30 females). Also,40 (33±12.077) healthy control. There was found a significant decrease (P< 0.001) in miRNA-223 levels in patients with lupus nephritis the mean fold change (0.11±0.22) compared to the controls (1.02±0.188). miRNA-223 was significantly down-regulated (P < 0.001) in patients with lupus arthritis (0.16±0.229) compared to the controls (1.02±0.188). According to gender, there is a difference in microRNA- 223 levels between males and females, and there is a significant association with gender (P<0.001) in lupus nephritis, mean fold change in males and females (0.34 ± 0.28) and (0.007±0.003) respectively, while in lupus arthritis mean fold change (0.36±0.15) and (0.1±0.04) in males and females respectively with (P<0.001). The cases are divided into four groups according to age, into four age groups, miR-223 levels significantly correlated with age, (P=0.028) in lupus nephritis and (P=0.01) in lupus arthritis. The sensitivity and specificity of miRNA-223 were 100% in lupus nephritis, The Receiver operating characteristic (ROC) curve showed (AUC) was (1.000) and a cut-off point of (0.6716). Also, the sensitivity and specificity of microRNA-223 in lupus arthritis were 100%. ROC observed (AUC) was (1.000) and cut off (0.6933).