Bachground: Polycystic ovary syndrome (PCOS) is a common condition that often leads to metabolic complications such as insulin resistance (IR) and obesity. Visfatin is a peptide that plays a role in endocrine, autocrine, and paracrine functions. Methods: Samples were collected from 90 women aged 16 to 40 years, divided into two groups. The PCOS group comprised 60 samples, with 30 women who had insulin resistance and 30 women who did not. The healthy group included 30 samples. The visfatin levels for all participants and performed genotyping for specific regions of the visfatin (rs1330082) gene using Real-time PCR were measured. The statistical significance is defined as p ≤ 0.05 for all comparisons. Results: The results showed that women with PCOS and insulin resistance had significantly higher levels of serum visfatin compared to other studies (6.640±0.3886, p <0.0001). However, there was no significant difference regarding the frequency of visfatin rs1330082 allele between the study groups. Conclusion: The research indicates that measuring serum visfatin levels could be a helpful tool for predicting insulin resistance in women with PCOS. However, we did not find any significant correlation between visfatin allele frequencies (rs1330082) and susceptibility to PCOS when compared to healthy controls.