Many plants around the world suffer significantly from plant parasitic nematodes, so transgenic crops were suggested as an application of biotechnology to make plants more resistant to nematodes in a safe way and without causing any changes in the ecosystem of plants. There are a few strategies for controlling nematodes in cucumber, and one of these methods uses natural resistance genes (R-genes) to resist nematodes. Our study was conducted with an isolated mi gene from nematode-resistant tomato plants, which produce proteins capable of preventing nematode feeding and detected with the PCR method then the resistant gene was inserted into a pEGAD vector and carried out in chitosan nanoparticles to be transformed into plants (cucumber cultivars) using Injections. The transgenic plants were tested by using spore suspension on a leaf. As a result of this study, a UV/visible spectroscope was used to characterise chitosan nanoparticles, and GFP gene florescent light found in transgenic plants compared to control. This will be a new approach to controlling nematodes, not only with genetic engineering but also with chitosan nanoparticles to get new cucumber cultivars to resist nematodes.