Objective several females fail to conceive after several attempts. This is an indication of a defect in fertility, based on several of studies. The ability of hormones as an indicator in the assessment of infertility has received a lot of attention. Methods, the study included 76 female who suffered from reproductive problems and who were previously diagnosed with infertility, and 50 healthy as a control group, age range of 22-40 years and each group of females subclassification according to BMI. Samples were collected from women attending the infertility center in Al-Sadr Medical City in Al-Najaf. Results , the current study aimed to study the most important hormones associated with infertility in women and link the relationship between them. Study of hormone levels LH, FSH and PRL correlation, the distinction between normal-weight and obese individuals, that obesity had an effect on LH, PRL and FSH levels, there was a significant difference in the means of all groups, with the obese patient and control group. Conclusion, the levels of prolactin and LH were better in the serum of unproductive women compared to healthy women.