Metallo-lactamases (MBL) have the ability to hydrolyze,  carbapenems, which are crucial medications used to treat Pseudomonas aeruginosa infections, To effectively treat infections brought on by this bacterium, quick detection of the MBL-producing Pseudomonas aeruginosa is required. In this study, Polymerase chain reaction (PCR) analysis was used to examine the prevalence of MBL-producing Pseudomonas aeruginosa in burn and non-burn patients, 68 Pseudomonas aeruginosa isolates were collected from different clinical sources from two hospitals in Baghdad. Antibiotic susceptibility tests were PCR performed on carbapenem-resistant isolates, Among the Pseudomonas aeruginosa isolates, 50% were meropenem and 39% were imipenem, Among all Pseudomonas aeruginosa isolates, 63.23% were multidrug-resistant and 51.5% were resistant to carbapenems (meropenem and imipenem), It has been determined blaVIM, blaSPM and blaNDM in 22.9%, 2.9% and 5.7% respectively blaIMP were not observed. For the efficient treatment of carbapenem-resistant P. aeruginosa infections, epidemiological and geographic evaluation of MBL-producing P. aeruginosa should be taken into consideration.