The current study aims to detect the gene expression of genes (CCL3, miRNA-b) in Otitis Media patients and healthy persons. This finding included 50 samples collected from healthy subjects and 100 samples from a patient suffering from otitis media who attended Al-Sadr Medical City (ENT Department) in Al-Najaf Governorate during the period from February 2022 to June 2022. The samples had an average age ranging from 5 to 70 years. In this case-control study, the gene expression of these genes was compared in people with Otitis media and healthy people. PCR technique is used. The patient and control groups' samples were amplified using polymerase chain reactions. The results of the molecular study (gene expression) showed a high significant increase in the level of gene expression in patients for the two genes CCL3, miRNA146-b (9.807 ± 1.14 (6.405 ± 0.7576), respectively, compared to the control group, with a high significant difference at P≤0.05.