The study aimed to evaluate the serum levels of the ATPO, ATG, and TRAB in patients with patients with autoimmune thyroiditis such as Hashimoto thyroiditis and Graves disease,  and in control groups. The results of current study showed the concentrations of thyroid hormones T3, T4, TSH, FT3, FT4 were measured to determine the patients who have an underactive thyroid gland or have hyperthyroidism and compared them to a control group, where it was found that the patients who had an underactive thyroid had significantly decreased levels of T3, T4, FT3 and FT4 concentrations, while their TSH level increased significantly than the rest of the patients and the control group. Most of the patients' samples were patients who had hypothyroidism. After that, special tests were done to detect the presence of the specific antibodies associated with this disease (AITD), which are ATPO, ATG, and TRAb, in addition to the clinical diagnosis of patients by the physician using the ultrasound device. It was found that patients with Graves' disease had a significant increase in the level of TRAb concentration compared to Hashimoto's patients and healthy controls, while the concentration of ATG was significantly increased in patients with Hashimoto's disease compared to patients with Graves' disease and healthy controls, and the concentration of ATPO was significantly elevated in both patients with Graves' and Hashimoto's disease compared to healthy controls.