The current study aims to create a new spectroscopic method for measuring sulphanilamide in large quantities and in pharmaceutical form. The proposed method involves using hydrogen peroxide as an oxidizing agent to oxidize sulphanilamide and P-aminophenol. Then, with the help of the peroxidase enzyme, an oxidative coupling reaction takes place between the two oxidation process byproducts to produce a brightly colored molecule with an absorbance value of 511 nm. To get the highest color intensity possible, reaction conditions are tuned. The absorbance was discovered to rise linearly with sulphanilamide content. The rules adhere to the beer base's (2-36 g/ml) range. It was discovered that the correlation coefficients were 0.9984. According to calculations, the sandal's sensitivity is 0.035 g/cm2.These approaches' analysis and recovery investigations have been statistically validated. This technique is used to create pharmaceutical substances. Relative standard deviation ratio is 0.135. The accuracy was assessed using recovery experiments, and the findings revealed that the values were 102.66, 100.56, and 100.75. The methods employed are precise, simple, and repeatable.