Klebsiella pneumoniae is one of the bacterial species with high antibiotic resistance. The present study aimed to investigate the effect of Minimal Inhibitory Concentration (MIC) of Ceftriaxon and Gentamicin on the antibiotic pattern of multi-drug resistant K. pneumoniae isolated from urinary tract infections. Sixty K. pneumoniae isolates have been isolated from 300 urine specimens collected from patients with urinary tract infections. Vitek 2 Compact System was used for identification and detection of antibiotic resistance patterns of bacterial isoltes. The highest percentage of antibiotic resistance of bacterial isolates to ampicillin (100%), followed by cefazolin (53.3%), while the lowest percentage of resistance was shown to Imipenem and tigecycline, which were 5% and 3.3%, respectively.  The effect of MIC of gentamicin and ceftriaxon toward 10 selected isolates were used to select mutated isolates, then the effect of both antibiotic-on-antibiotic resistance of mutated isolates was detected using Vitek 2 Compact System. The results of the mutation experiment using ceftriaxone gave successful results to 7 isolates while 5 isolates gave positive results to gentamicin. The results of antibiotic resistance pattern of mutated isolates showed that all mutated isolates became resistance to most tested antibiotics in comparison with original isolates. We concluded that indiscriminate and wrong use of antibiotics would lead to emergence of MDR isolates not only for antibiotics belonging to the same class but also to other classes.