On dairy farms, bovine respiratory disease (also known as BRD) is still considered to be one of the most serious infectious diseases. The main bacteria that cause BRD are Pasteurella multocida and Mannheimia hemolytica. Objectives: This research evaluated antibiotic resistance prevalence. M. hemolytica and P. multocida were isolated from bovine lung samples at Veterinary Farms and investigated for antibiotic resistance. Methods: Samples were taken from the lungs of both sick and healthy cattle. Using bacteriology culture tests, testing of biochemicals, and the polymerase chain reaction (PCR) method, both Pasteurellaceae species were separated from the samples. The isolates were identified using the PCR methodology. The Kirby–Bauer disc diffusion method was used to find out how susceptible the isolates are to antibiotics. RESULTS: 120 samples were obtained and P. multocida 60 (50%) and M. hemolytica strains 30 (20%) were found in the samples' bacteriological analysis. M. haemolytica from pneumonia samples differed from those from apparently healthy lung tissues. Penicillin and erythromycin were the most resistant, while all isolates were sensitive to tulathromycin. Pasteurellaceae species were resistant to multiple drugs. Conclusions: BRD pathogens must be monitored for antibiotic resistance patterns to update treatment strategies. To establish resistance-limiting measures, surveillance studies are needed.