Background: Infection with the hepatitis B virus is a major global health concern. In Peshawar, HBV infection is widespread and affects all demographics, especially pregnant women. Objective: The purpose of this research is to determine how common HBV is, as well as the risks and complications associated with various clinical parameters and patient demographics. Methodology: 200 patients were reported and tested with enzyme-linked immunosorbent assay (ELISA) for HBeAg and immunochromatographic assay for HBs-Ag (ICT). For quantifying HBV DNA, an RT-PCR assay was performed. In-person interviews with study participants were conducted using the research software Performa. Results: In our study, a total of 200 patients were investigated for HBV infection in KP, including Peshawar, Charsadda, Kohat, Waziristan, and Mardan region.  According to our study, the highest prevalence was found in Peshawar with 30% (60/200), Charsadda (10%, 20/200), Kohat (7.5%, 15/200), Mardan (20%, 40/200), Waziristan (12.5%, 25/200). Mardan had the second greatest incidence, at 20.2% (40/200). The current study indicated a prevalence of 21.3% for affected family members based on relation, including 9.3% for spouses, 2.7% for mothers, 4% for siblings, and 5.3% for children. HBV patients' ALT, bilirubin, creatinine, and ultrasonography levels indicate treatment, severity, and antiviral medicine efficacy. 200 HBV patients were monitored for these clinical indicators after entecavir and tenofovir treatment. Patients with normal ALT were 81.3% (162/200) (87.3%), 18.7% (38/200) with high, and 20/200 (10.4%) with raised creatinine. After antiviral treatment, patients had normal bilirubin 93.3%. The study assessed 64% and 36% of patients on tenofovir and entecavir for different durations. Due to renal failure and diabetes, HBV-infected patients had a 1.3% death rate. Conclusion: Males get infected more often than females since they're more exposed to the environment. Due to intimate contact and little awareness, HBV prevalence was highest in spouses with a family history.