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282301

Screening and Optimization of Alkaline Protease Production from Bacteria Isolated from Seawater in The North West of Algeria

Article

Last updated: 24 Dec 2024

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Abstract

Bacteria are attracting the interest of worldwide investors; their use is interesting in several industrial fields, this organism produces a wide variety of extracellular enzymes, including proteases. The objective of this study was to evaluate the production of protease by different bacterial strains isolated from local marine samples collected from the Cap Rousseau beach in the Oran city, northwestern Algeria, 44 bacterial isolates were tested for protease production by cultivating them on skim milk agar medium.  The proteolytic activities of all strains were tested using skim milk agar and gelatin agar. Among the 14 isolates that showed a significant hydrolysis diameter, two bacterial strains EC23 and EC2S3 demonstrated the highest potential for protease production and they were selected for further studies. In addition, the extracellular protease was examined using the fermentation production medium at 30°C for 48h, with a constant agitation of 150 rpm. The enzyme activity was determined under varying conditions of pH, incubation temperature, and salt concentration, using Sigma's Universal Protease Activity Assay. The enzyme from EC23 strain showed higher activity in all cases than the EC2S3 strain, which indicated that it was the most ideal organism for enzyme production.

DOI

10.21608/eajbsc.2023.282301

Keywords

Bacteria, Alkaline protease, enzyme activity, hydrolysis, identification, Optimization

Authors

First Name

Nourine,

Last Name

Zeyneb

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

zeyneb.nourine@univ-sba.dz

City

Algeria

Orcid

-

First Name

El Kadi

Last Name

Zohra

MiddleName

Fatima

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

elkadifatimazohra@yahoo.fr

City

Algeria

Orcid

0000-0002-9671-8505

First Name

Didaoui,

Last Name

Hayat

MiddleName

-

Affiliation

Biology Department, Science Faculty. Dr Moulay Tahar University of Saida Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Kanoun,

Last Name

Khedoudja

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Bouchouicha,

Last Name

Sara

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

sara.bouchouicha@univ-sba.dz

City

Algeria

Orcid

-

First Name

Bouyakoub,

Last Name

Nesrine

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

bou.nesrine@outlook.fr

City

Algeria

Orcid

-

First Name

Benine,

Last Name

Lamine

MiddleName

Mohamed

Affiliation

Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Mouffok,

Last Name

Benali

MiddleName

-

Affiliation

Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Harir,

Last Name

Noria

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Abbouni,

Last Name

Bouziane

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Megharbi,

Last Name

Aicha

MiddleName

-

Affiliation

Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

Volume

15

Article Issue

1

Related Issue

38909

Issue Date

2023-06-01

Receive Date

2022-11-28

Publish Date

2023-01-28

Page Start

85

Page End

96

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 15_Issue 1_Pages 85-96.pdf

PDF . 973.6kB

Link

https://eajbsc.journals.ekb.eg/article_282301.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=282301

Order

282,301

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Screening and Optimization of Alkaline Protease Production from Bacteria Isolated from Seawater in The North West of Algeria

Details

Type

Article

Created At

24 Dec 2024