Background:  Scientific research has shown that infertile males exhibit more pronounced impairment in sperm quality and higher levels of oxidative stress. Elevated levels of reactive oxygen species have a detrimental effect on the rigidity, motility, and DNA repair capacity of sperm. It is noteworthy that the levels of reactive oxygen species in the body have a significant impact on male fertility, specifically in relation to acrosome response and activation. Methods: This study comprised two groups, each comprising 50 couples who were experiencing infertility. Group 1: couples who were unable to conceive due to male-factor infertility. Group 2:  couples who are facing infertility, with smoking and the participation of the male partner being identified as contributing factors. Before undergoing intracytoplasmic sperm injection, all male partners received antioxidant therapy in the form of FERTITONE X pills. The study participants provided seminal fluid samples at the beginning of the experiment and then again after a period of three months. The evaluated metrics encompassed semen parameters, including sperm count, motility, viability, morphology, and DNA fragmentation index. Results: Administering FERTITONE X treatments has been observed to improve various sperm parameters, such as count, motility, and viability (p < 0.001). Furthermore, enhancements in sperm morphology and DNA fragmentation index have been noted across all groups (p < 0.001). Conclusion:  In conclusion, antioxidant therapy improves semen parameters, as well as its influence on the results of in vitro fertilization and intracytoplasmic sperm injection procedures.