Cancer is a significant reason for death globally, emerging as the second most common cause of mortality worldwide. Ehrlich ascites carcinoma (EAC) cells are investigational tumor models used globally in cancer research. Radiation therapy is commonly applied to cancerous tumors because of its ability to control cell growth. This study aims to explore the potential therapeutic effects of Low Doses of Gamma Irradiation (γ-radiation) on Ehrlich carcinoma-bearing mice. Forty adult female Swiss albino mice were divided into four groups: Normal control group (NC) of 10 mice were given saline; Ehrlich carcinoma group (EC) of 10 mice were intramuscularly inoculated with 0.2ml of EAC containing 2.5x10⁶ in the right thigh of the lower limb of female mice; Tumor-bearing EC-Irradiated group (EC-IR) of 10 mice were exposed to 0.5Gy of gamma radiation two times weekly for 2 weeks; Radiation group (IR) of 10 mice were exposed to 0.5 Gy of gamma radiation two times weekly for 2 weeks. The current result showed a significant increment in muscle size of the right thigh, MDA, H₂O₂, TNF-α, IL-6, VEGF, WBC, and Platelets (PLT). Also, EC causes a reduction of antioxidant enzymes (GSH), RBCs and Heamoglobin (Hb). However, the therapeutic group (EC-IR) showed an improvement in biochemical and hematological parameters.