Two derivatives of 2-Benzoylpyridine namely; (E)-4-((E)-4-((E)-phenyl(pyridin-2-yl)methyleneamino)benzyl)-N-(phenyl(pyridin-2-yl)me-thylene)benzenamine (L) and its Cu(II) complex were prepared and screened in vitro on Eobania vermiculata species. The assessment of LC₅₀ values of screened chemicals after 3 days of treatments was performed via the contact method. A series of four concentrations of the investigated compounds; (50, 150, 250, and 350 ppm) was prepared by using a mixture of distilled water and DMSO (3:1) by volume. The obtained results demonstrated that the tested snails were strongly affected after treatments with the prepared chemical compounds, which reflected the high toxicity of those compounds. LC₅₀ data of (E)-4-((E)-4-((E)-phenyl(pyridin-2-yl) methyleneamino) benzyl)-N-(phenyl(pyridin-2-yl) methylene) benzenamine and copper chelate are 54.81 and 91.29 ppm, respectively. Biochemical investigations for the treated snails were carried out to estimate the total protein content (TP) as well as the activity of the liver (ALT) and (AST) enzymes after 72 h from starting the exposure. The data authenticated that the (L) and Cu (II) complex reduced the AST activity to (53.97 ± 1.06 U/L) and (42.93 ± 0.96 U/L), respectively, compared to (56.97 ± 0.72 U/L) for control. While a high increase in ALT activity was noticed after treatment with the two tested compounds. The (TP) content of the tested snails decreased from (22.11 ± 0.22 mg/g) in control to (21.11 ± 0.80 mg/g) and (18.43 ± 0.71 mg/g) in the (L) and Cu (II) complex, respectively.