Regarding the fast development of nanotechnology and its various applications, the present study was carried out to estimate the potential toxic effect of silver nanoparticles (AgNPs) on the fertility of male albino mice. Thirty-five adult male mice were randomly divided into 5 groups (7 mice per group) as the following: the first group (GP₁) was the control group which was orally administered distilled water. The second group (GP₂) and the third group (GP₃) were orally administrated silver nanoparticles (62.5 mg/kg/d & 125 mg/kg/d, respectively) for 35 days. The fourth group (GP₄) and the fifth group (GP₅) were orally administrated silver nanoparticles (62.5 mg/kg/d & 125 mg/kg/d, respectively) for 35 days then they were left 15 days without treatment for natural recovery and they were allowed to mate. Changes in the body or testicular weights or gonadosomatic index were recorded. Sperm analysis (sperm viability, motility, count and morphology) and damage in epididymal sperm DNA (comet assay) also were investigated. The results confirmed that silver nanoparticles have a toxic effect on male mice testis that leads to male infertility with high doses by reducing the number of sperms and increasing abnormalities in sperm motility and morphology.