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353943

Isolation of Proline-Rich Protein Gene from Potato under Drought Stress

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Last updated: 24 Dec 2024

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Abstract

Drought stress is considered one of the main factors that reduce plant growth, development and production. Therefore, studying plants during drought stress is highly useful to discover the newly gained characteristics of plants. The potato is considered one of the most important crop with economic value in Egypt and over the whole world. The main target is the proline-rich protein extracted from potato plants under drought stress. A stress experiment was applied to two potato (Solanum tuberosum L.) cultivars Desiree and Diamante. The stress experiment was conducted by poly-ethylene glycol (PEG) 4000; the used water potentials were: zero (control), (PEG 2%), (PEG 4%) and (PEG 8%), after drought stress, the solutes accumulation in the two potato cultivars were determined. Increasing drought stress through elevating PEG decreased leaf area as well as shoot and root lengths. Incontorary, the chlorophyll and proline contents increased with increasing PEG treatments. The proline-rich protein gene was cloned to the pGEM-T Easy vector and was submitted to the gene bank to be used later for enhancing drought resistance in other cultivars.

DOI

10.21608/ajs.2024.203689.1519

Keywords

Drought stress, polyethylene glycol (PEG), Proline-rich protein gene, Potato cultivars

Authors

First Name

Heba

Last Name

Mohammed

MiddleName

Sayed

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

Email

hebamohammed48@yahoo.com

City

Giza

Orcid

0009-0003-7847-7511

First Name

Mohamed

Last Name

Abdel-Salam

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University,P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

rashed@agr.asu.edu.eg

City

-

Orcid

0000-0002-6815-6306

First Name

Ayman

Last Name

Hanfy

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University,P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

aymanatta@agr.asu.edu.eg

City

-

Orcid

-

First Name

Mervat

Last Name

Diab

MiddleName

Ragab

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

Email

mervatdiab10@gmail.com

City

-

Orcid

0000-0001-7882-5385

First Name

Nouh

Last Name

Ahmed

MiddleName

Eid

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University,P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

egynouh@agr.ash.edu.eg

City

-

Orcid

-

First Name

Emad

Last Name

Metry

MiddleName

Anis

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

Email

anisemad@yahoo.com

City

Cairo

Orcid

-

Volume

32

Article Issue

1

Related Issue

47481

Issue Date

2024-06-01

Receive Date

2023-04-02

Publish Date

2024-06-01

Page Start

63

Page End

73

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 32_Issue 1_Pages 63-73.pdf

PDF . 606.9kB

Link

https://ajs.journals.ekb.eg/article_353943.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=353943

Order

353,943

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

Isolation of Proline-Rich Protein Gene from Potato under Drought Stress

Details

Type

Article

Created At

24 Dec 2024