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353942

Glutathione-S-Transferase Response Towards Imidacloprid in Honeybees (Apis mellifera L.)

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Last updated: 03 Jan 2025

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Abstract

Imidacloprid is a systematic neonicotinoid widely used to combat piercing-sucking insects; however, neonicotinoids, despite having low effects on vertebrates, showed high adverse effects on honeybees (Apis mellifera L.). Glutathione-S-transferases (GST) are an important constituent of the defense system in detoxifying invading chemicals. This work examines the effects of imidacloprid on GST activity in vivo and in vitro conditions. Results revealed that there are two responses of GST activity toward IMI treatment; first, stimulation of enzyme activity to combat and detoxify the insecticide. Second, direct inhibition of GST, which is confirmed by the in vitro inhibition with IC50 887.42 ppm. At a short exposure time to IMI (2 hours) at an IMI concentration of 0.35 ppm, the enzyme was stimulated up to 113% while increasing exposure time or IMI concentration, the inhibition effect dominates.

DOI

10.21608/ajs.2024.238333.1534

Keywords

GST, neonicotinoid, insecticide, Insect resistance, in vivo, in Vitro

Authors

First Name

Hussein

Last Name

Ali

MiddleName

M

Affiliation

Agricultural Biochemistry Dept, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

hussein_galaleldeen@agr.asu.edu.eg

City

Cairo

Orcid

0000-0002-6524-0780

First Name

Basma

Last Name

Abdel-Aty

MiddleName

-

Affiliation

Plant Protection Deptartment, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

basma_abdelaaty_na@agr.asu.edu.eg

City

-

Orcid

-

First Name

Walaa

Last Name

El-Sayed

MiddleName

-

Affiliation

Plant Protection Deptartment, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

walach2000@yahoo.com

City

-

Orcid

-

First Name

Faiza

Last Name

Mariy

MiddleName

M

Affiliation

Plant Protection Deptartment, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

faizamariy@gmail.com

City

-

Orcid

-

First Name

Gamal

Last Name

Hegazy

MiddleName

M

Affiliation

Plant Protection Deptartment, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

gamalhegazy@gmail.com

City

-

Orcid

-

Volume

32

Article Issue

1

Related Issue

47481

Issue Date

2024-06-01

Receive Date

2023-09-23

Publish Date

2024-06-01

Page Start

1

Page End

6

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 32_Issue 1_Pages 1-6.pdf

PDF . 166.1kB

Link

https://ajs.journals.ekb.eg/article_353942.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=353942

Order

353,942

Type

Short Communication

Type Code

1,582

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

Glutathione-S-Transferase Response Towards Imidacloprid in Honeybees (Apis mellifera L.)

Details

Type

Article

Created At

24 Dec 2024