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330124

Molecular Genetic Studies on Honeybees in Response to Some Environmental Stresses

Article

Last updated: 24 Dec 2024

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Abstract

The honeybee, Apis mellifera, is a widespread pollinator insect. Colony collapse disorder (CCD) has recently caused a significant decline in honeybee numbers worldwide. The beekeeping industry, agriculture, and world biodiversity are threatened by the honeybee population decline. We examined the mRNA levels of heat shock protein gene 60 (hsp60), acetylcholinesterase 1, and acetylcholinesterase 2, as biomolecular markers of the insect response to three separate forms of environmental stress, including high temperature, low temperature, and insecticide. The obtained results indicated increased levels of hsp60 and acetylcholinesterase 2 after the exposure of worker bees to temperatures of 17, 32, and 40°C for one hour, as well as exposure to a low dose of pesticide for 24 h ingestion of imidacloprid solution (2 ppm); meanwhile, acetylcholinesterase 1 showed remarkable expression decrease under heat and cold stresses but increased under pesticide treatment.  The responses of honeybees to hsp60, acetylcholinesterase 1, and acetylcholinesterase 2 are diverse and very specific to various stimuli. According to this research, the distinct expression profiles of hsp60 and acetylcholinesterase 2 can serve as useful instruments for tracking honeybees' vulnerability to varied environmental effects. Due to its insufficient responsiveness, acetylcholinesterase 1 expression is not permitted for use as a biomarker.

DOI

10.21608/ajs.2023.211527.1521

Keywords

Honeybees, molecular markers, Heat Shock Protein 60, Acetylcholinesterase 1, Acetylcholinesterase 2, Imidacloprid

Authors

First Name

Rehab

Last Name

Mohamed

MiddleName

Afify

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

rehabafify@agr.asu.edu.eg

City

Qalibiya

Orcid

0000-0001-9986-587X

First Name

Naglaa

Last Name

Ebeed

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

nebeed@agr.asu.edu.eg

City

-

Orcid

0000-0002-1527-8000

First Name

Khalid

Last Name

Fahmy

MiddleName

Ibn Elwaleed

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

khalid.fahmy@agr.asu.edu.eg

City

-

Orcid

-

First Name

Naima

Last Name

Alsenosy

MiddleName

Koutb

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

neima_koutb@agr.asu.edu.eg

City

-

Orcid

-

First Name

Sawsan

Last Name

Abdelmegeed

MiddleName

mohamed

Affiliation

Plant Protection Department, Faculty of Agriculture, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

sawsan_ali@agr.asu.edu.eg

City

cairo

Orcid

0000-0002-3027-3566

Volume

31

Article Issue

2

Related Issue

45001

Issue Date

2023-12-01

Receive Date

2023-05-21

Publish Date

2023-12-01

Page Start

265

Page End

272

Print ISSN

1110-2675

Online ISSN

2636-3585

Link

https://ajs.journals.ekb.eg/article_330124.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=330124

Order

330,124

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

Molecular Genetic Studies on Honeybees in Response to Some Environmental Stresses

Details

Type

Article

Created At

24 Dec 2024