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330123

Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9

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Last updated: 03 Jan 2025

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Abstract

Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.  

DOI

10.21608/ajs.2023.203532.1518

Keywords

CRISPR-Cas9, amylopectin, granule-bound starch synthase, sgRNAs, Agrobacterium- mediated transformation

Authors

First Name

Nermin

Last Name

Ali

MiddleName

Mohamed

Affiliation

Department of Plant Genetic Transformation,Agricultural Genetic Engineering Research Institute - Agricultural Research Center, Giza, Egypt.

Email

nona_20062007@hotmail.com

City

cairo

Orcid

0000-0002-7164-5792

First Name

Mohamed

Last Name

Abdel-Salam

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University. Cairo, Egypt

Email

rashed@agr.asu.edu.eg

City

-

Orcid

0000-0002-6815-6306

First Name

aiman

Last Name

hanfy

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

aymanatta@agr.asu.edu.eg

City

-

Orcid

-

First Name

Haytham

Last Name

Abd-Elhalim

MiddleName

Mohamed

Affiliation

School of Biotechnology, Badr University in Cairo, Badr City, 11829, Cairo, Egypt

Email

haytham.mohamed@ageri.sci.eg

City

Cairo

Orcid

-

First Name

Nouh

Last Name

Ahmed

MiddleName

Eid

Affiliation

Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

egynouh@agr.ash.edu.eg

City

-

Orcid

-

First Name

Emad

Last Name

Metry

MiddleName

Anis

Affiliation

Department of Plant Genetic Transformation,Agricultural Genetic Engineering Research Institute - Agricultural Research Center, Giza, Egypt.

Email

anisemad@yahoo.com

City

Cairo

Orcid

-

Volume

31

Article Issue

2

Related Issue

45001

Issue Date

2023-12-01

Receive Date

2023-04-01

Publish Date

2023-12-01

Page Start

173

Page End

184

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 31_Issue 2_Pages 173-184.pdf

PDF . 680.8kB

Link

https://ajs.journals.ekb.eg/article_330123.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=330123

Order

330,123

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

Increasing of Amylopectin in Tetraploid Potato Desiree Cultivar (Solanum tuberosum L.) Via CRISPR/Cas9

Details

Type

Article

Created At

24 Dec 2024