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294298

Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata

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Last updated: 24 Dec 2024

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Abstract

Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.

DOI

10.21608/ajs.2023.162834.1498

Keywords

Ephedra alata, cell suspension culture, Callus culture, Ephedrine, pseudoephedrine

Authors

First Name

Naglaa

Last Name

Abdelsalam

MiddleName

Abdallah

Affiliation

Tissue Culture Unit, Department of Genetic Resources , Desert Research Center , El-Matareya , Cairo, Egypt.

Email

naglaaa.abdallahh@gmail.com

City

-

Orcid

0000-0002-0338-0665

First Name

mona

Last Name

Abd El-Aal

MiddleName

-

Affiliation

Agricultural Botany Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

mona_eldars@agr.asu.edu.eg

City

-

Orcid

-

First Name

mohamed

Last Name

Aboul Fotouh

MiddleName

-

Affiliation

Biochemistry Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

mohamed_mesalhi@agr.asu.edu.eg

City

Cairo

Orcid

0000-0002-7449-2226

First Name

mohamed

Last Name

Reda

MiddleName

-

Affiliation

Tissue Culture Unit, Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt.

Email

reda.mhamed60@gmail.com

City

-

Orcid

-

First Name

Ibrahim

Last Name

Safe

MiddleName

-

Affiliation

Agricultural Botany Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt

Email

ibrahim_mfm@agr.asu.edu.eg

City

-

Orcid

0000-0003-1601-5082

Volume

31

Article Issue

1

Related Issue

39978

Issue Date

2023-06-01

Receive Date

2022-09-14

Publish Date

2023-06-01

Page Start

101

Page End

109

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 31_Issue 1_Pages 101-109.pdf

PDF . 392.9kB

Link

https://ajs.journals.ekb.eg/article_294298.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=294298

Order

28

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata

Details

Type

Article

Created At

24 Dec 2024