The domestic mosquito Culex pipiens (Diptera: Culicidae) is one of the most important public health pests spread in Egypt, so we studied biochemical and toxicological changes to traditional and bio-pesticides on the third instar larvae of the domestic mosquito. Three organic pesticides (chlorpyrifos, lambada cyhalothrin, imidaclopride) and two biopesticides (emamectin benzoate, spinosad) at different concentrations treated on larvae and calculating the value of   LC_{50 after} treatment and the rate of resistance were studied. The results showed that the South strain of domestic mosquito larvae were high resistance against chlorpyrifos ethyl, it was found that the most efficient tested pesticide was spinosad, where it was in the South strain 55,227-fold, and in the East strain, The relative potency of imidacloprid was 12.17-fold and in the West strain, imidacloprid was the first category, followed by spinosad. Activity acid phosphatase, alkaline phosphatase, acetylcholinesterase and glutathione -s- transferase in larvae, were determined the highest activity in South strain indicated that this strain had the highest resistance.