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318234

Molecular Detection of Upper Respiratory Tract Viral Infection with RSV, HPIV & Adenovirus among SARS-CoV2 Negative patients with respiratory illness admitted in tertiary care ho

Article

Last updated: 03 Jan 2025

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Abstract

Background: The most prevalent disease-causing agents in humans are respiratory viruses, which have a global influence on morbidity and death. The Adenovirus, respiratory syncytial virus (RSV), and parainfluenza viruses (HPIV) are the dominant respiratory viruses that circulate most often throughout all continents. Vaccines and potent antivirals are not yet available, but progress has been made in understanding their biology and the core problems of the host-parasite interaction. Aims & Objective: To study respiratory viral infections among clinical manifestations among COVID-19 negative patients with pseudo symptoms. Materials & Methods: This cross-sectional hospital-based study conducted on VRDL, Burdwan Medical College where we used real-time PCR for SARS CoV2 screening and viral DNA extraction for Adenovirus screening. Real-time PCR through SyBr green was used to detect RSV, HPIV, and Adenovirus in 120 clinical samples. Beta Actin was used as a test control for DNA compatibility for PCR amplification. Result: 120 patients were studied, with 13 being Adenovirus positive, three RSV positive, and two HPIV positive. 35% were infected with Adenovirus. RSV and HPIV also caused infection, with prevalence rates of 5.83% and 14.17%, respectively. Triple-infection was observed in 1 neonatal patient among 120. Conclusion: Triple infections in RSV, HPIV, Adeno, and neonates were found, but none of the 120 SARS-CoV2 infections were mono-infections. This suggests that respiratory viral infections among peudo-COVID19 signs and symptoms may lead to future pandemic, and further research is needed to confirm this.

DOI

10.21608/aps.2023.214314.1124

Keywords

Adenovirus, RSV, HPIV, RT-PCR, Clinical Manifestations

Authors

First Name

Nivedita

Last Name

Mukherjee

MiddleName

-

Affiliation

ICMR-DHR Viral Research & Diagnostic Laboratory, Burdwan Medical College, Burdwan, WB, India.

Email

mukherjeenivedita0@gmail.com

City

Burdwan

Orcid

-

First Name

Swati

Last Name

Nayek

MiddleName

-

Affiliation

Department of Pathology, Burdwan Medical College, Burdwan, WB, India.

Email

swatinayek.sn@gmail.com

City

-

Orcid

-

First Name

Sanjoy

Last Name

Mondal

MiddleName

-

Affiliation

Department of Orthopaedics, Burdwan Medical College and Hospital, Burdwan, WB, India.

Email

sanjoymondal5212.sm@gmail.com

City

Burdwan

Orcid

-

First Name

Tanusri

Last Name

Biswas

MiddleName

-

Affiliation

Department of Microbiology, Burdwan Medical College, Burdan, WB, India.

Email

dr.tanusribiswas@rediffmail.com

City

-

Orcid

-

First Name

Arghya

Last Name

Nath

MiddleName

-

Affiliation

ICMR-DHR Viral Research & Diagnostic Laboratory, Burdwan Medical College, Burdwan, WB, India.

Email

arghyanath.biotech@gmail.com

City

Burdwan

Orcid

-

Volume

7

Article Issue

2

Related Issue

43740

Issue Date

2023-12-01

Receive Date

2023-05-30

Publish Date

2023-12-01

Page Start

231

Page End

239

Print ISSN

2356-8380

Online ISSN

2356-8399

Link

https://aps.journals.ekb.eg/article_318234.html

Detail API

https://aps.journals.ekb.eg/service?article_code=318234

Order

318,234

Type

Original Article

Type Code

657

Publication Type

Journal

Publication Title

Archives of Pharmaceutical Sciences Ain Shams University

Publication Link

https://aps.journals.ekb.eg/

MainTitle

Molecular Detection of Upper Respiratory Tract Viral Infection with RSV, HPIV & Adenovirus among SARS-CoV2 Negative patients with respiratory illness admitted in tertiary care ho

Details

Type

Article

Created At

24 Dec 2024