The efficiency of most cancer therapies and antibiotics is restricted due to several issues, including medication resistance and non-specific negative effects. Due to their tunable properties, hydrogels based on graphene derivative and silver nano-particles (Ag-NPs) have been employed to overcome these limitations. In this manuscript, we synthesized gelatin methacryloyl (Gel-MA) to combine with functional nano-materials. Reduced graphene oxide (rGO) was synthesized by utilising ascorbic acid as a green reductant to form a composite with Gel-MA without affecting its unique characteristics. The prepared rGO and nano-composite hydrogels were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscope (SEM), X-ray Diffraction (XRD), Fourier Transform Infra-Red Spectroscopy (FT-IR), UV–Visible Spectrophotometry (UV–Vis), Raman, and Energy Dispersive-X-ray (EDX) Spectroscopy. The ninhydrin assay was used to quantify the degree of methacrylation. We found that the nano-composite swelled up to 2444% due to the high porosity of the hydrogel. The results demonstrated that the nanocomposite hydrogel showed higher antimicrobial activities than virgin (Gel-MA). The cytotoxicity of Gel-MA/rGO and Gel-MA/rGO/Ag composites against human lung cancer (A549) cells was evaluated using MTT assay. The Gel-MA/rGO/Ag composite showed remarkable cytotoxicity with an IC50 of 27µg/ml towards A549 cells compared with 109µg/ml of Gel-MA/rGO.