Moringa oleifera is one of the world's most significant therapeutic plants, it is rich in many phytochemicals including polysaccharides. Natural polysaccharides have a wide range of beneficial therapeutic and health-promoting properties. The current study investigated the antimicrobial in a 96-well plate, antioxidant by DPPH scavenging assay and antibiofilm activities of both polysaccharides (MOLPs) extracted from M. oleifera leaves and crude extracts of leaves (MOLCEs)  by different solvents (methanol, 70% ethanol, ethyl acetate, and hot distilled water (80°C)). Results revealed that the antimicrobial activity of MOLPs against S. aureus, B. Subtilis, E. coli, and P. aeruginosa at 500mg/ml was 72.7%, 73.1%, 75.2%, and 74.4%, respectively. The antimicrobial activity of hot water crude extract ranged from 67% to 71% while other crude extracts were less effective. DPPH scavenging activity of MOLPs and MOLCEs was concentration-dependent, at 500 mg/ml it was 76.56% and 81.87% for MOLPs and hot aqueous crude extract, respectively. Other crude extracts were less effective. The biofilm inhibitory activity of MOLPs against S. aureus and E. coli was 62.56% and 65.65%, respectively while was 55.5% and 57.42% for hot water crude extract. Other MOLCEs by organic solvents (methanol, 70% ethanol and ethyl acetate) showed low inhibitory activity. These results confirmed the importance of polysaccharides as a bioactive agent that can be used as a promising therapeutic agent.