In over fifty countries, the second leading cause of death after cardiovascular disease is cancer. Indeed, the 10^thmost diagnosed cancer worldwide is bladder cancer, with half a million new reported cases and fifty percent of deaths per year. While cancer is primarily regarded because of host genetics and environmental influences, microorganisms are linked to 20% of human cancers. The microbiota of each individual is unique, undergoing development in childhood and adulthood and inhabiting the human body in various sites exposed to the external environment, including the gastrointestinal tract, oral cavity, genitourinary tract, and skin. The composition of the urinary microbiota can function as a host protection mechanism, preventing infections when the physiological balance is maintained or allowing infection and colonization by pathogens when the balance is disturbed by factors related to the host or the environment. It was previously assumed that healthy individuals had sterile urine using culture-based methods. However, using high-throughput DNA sequencing and improved culture techniques, numerous microorganisms are discovered in the urine of healthy people, many of which are not detectable by standard culture techniques. So, the symbiotic relationship between humans and commensal microbiota has become a topic of interest in both basic and clinical fields all over the world. This review will discuss the epidemiology and external risk factors of bladder cancer, as well as the association between urinary microbiota, urinary tract disorders, and bladder cancer, besides their role in treatment.