Climate change is a major threat to agricultural sustainability and productivity around the world. For this, modern breeding strategies have focused on improving crop qualities through crop wild relatives (CWRs). Increased genetic diversity and enhanced agricultural resilience through the utilization of crop wild relatives (CWRs), which are closely related to cultivated crops, offer a sustainable approach for crop development amidst ongoing climatic concerns. In order to provide effectively data in agronomic, nutritional, and breeding efficiency traits for crop advances, this study aims to provide some phytochemical proprieties and biochemical attributes for some cultivated plants and their wild relatives belonging to three families; Apiaceae, Asteraceae and Brassicaceae. The main and secondary metabolites of the chosen domesticated plants were differed from those of their wild counterparts, according to the results of the phytochemical screening. Wild Lactuca serriola (Asteraceae) has the highest nutrient density (457.21 K cal/ 100 g) and Apium leptophyllum has the lowest (381.94 Kcal/ 100 g). Comparing IC50 values, Daucus carota has the lowest (0.052 mg/ml) and Lactuca serriola has the greatest (0.457 mg/ml) antioxidant activity. Identification of the phytoconstituents and GC-MS analysis is one of the best and accurate techniques to nature of active principles in medicinal plants.