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387074

Serum and urinary galactose-deficient IgA1 as a tool for diagnosis of IgA nephropathy in pediatric patients with hematuria

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Pediatrics

Abstract

Background: IgA nephropathy (IgAN) is an immunopathologic diagnosis based on a renal biopsy. Diagnosis of IgAN may not be made in the milder cases or may be delayed until clinical manifestations are severe. We sought to assess the validity of serum and urinary galactose-deficient IgA1(Gd-IgA1) as a possible non-invasive diagnostic biomarker of IgAN. Methods: A cross-sectional study was conducted at Pediatric Nephrology Clinic, Children's Hospitals, Ain Shams University on 40 patients with recurrent gross glomerular hematuria diagnosed with renal biopsy and divided into two equal groups, group 1 patients with IgAN and group 2 with non-IgA glomerular diseases. Serum and urinary Gd-IgA1 levels were measured by ELISA using an anti-Gd-IgA1 monoclonal antibody (KM55). Laboratory investigations included complete blood count (CBC), erythrocyte sedimentation rate (ESR), complement 3 level in serum (C3), antinuclear antibody (ANA), anti-double strand DNA, urine protein/creatinine (UP/Cr) ratio, serum, and urinary total IgA levels (ELISA). Results: The study included 20 patients with IgAN, of which 13 were males and 7 were females, in addition to 20 patients with non-IgAN, of which 12 were males and 8 were females. In the IgAN group median age was 6.5 (5-9) years old. In the non-IgAN group, median age was 9 (4.5-13) years old. Serum Gd-IgA1 levels were significantly elevated in children with IgAN compared with children with non-IgA glomerular diseases (p-value 0.001). The serum Gd-IgA1 cut-off point to differentiate between IgAN and non-IgA glomerular diseases was 240 ng/ml with 75 % sensitivity and 80 % specificity and AUC 80.2%. There was no statistically significant difference between IgAN and non-IgA glomerular diseases regarding urinary Gd-IgA1 (p-value 0.08), Ptn/Creat ratio (p-value 0.055), serum and urine total IgA (p-value 0.144 and 0.288). Conclusion: Serum Gd-IgA1 level is higher in IgAN patients compared to non-IgA glomerular diseases, serum Gd-IgA1 may be used as a non-invasive diagnostic biomarker for IgAN patients.

DOI

10.21608/ejpa.2024.277144.1064

Keywords

Keywords: IgA nephropathy, Serum galactose-deficient IgA1, Urinary galactose-deficient IgA1, ELISA

Authors

First Name

Nour

Last Name

Abderlahman

MiddleName

Abdullah

Affiliation

Pediatric, faculty of medicine Ain shams university, Egypt

Email

nouraboaof@gmail.com

City

-

Orcid

-

First Name

Ihab

Last Name

El-Hakim

MiddleName

-

Affiliation

MD, PhD, AFSA. Professor of pediatrics and pediatric nephrology, Ain Shams University, Egypt.

Email

ihab.elhakim@gmail.com

City

-

Orcid

-

First Name

Neama

Last Name

Mohamed

MiddleName

L.

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

EL Farsy

MiddleName

S.

Affiliation

Department of Pediatrics, Faculty of Medicine, Ain Shams University

Email

mohamedelfarsy@med.asu.edu.eg

City

-

Orcid

-

Volume

22

Article Issue

2

Related Issue

50888

Issue Date

2024-10-01

Receive Date

2024-03-15

Publish Date

2024-10-01

Page Start

73

Page End

80

Print ISSN

1687-1642

Online ISSN

2314-8934

Article File

EJPA_Volume 22_Issue 2_Pages 73-80.pdf

PDF . 302.1kB

Link

https://ejpai.journals.ekb.eg/article_387074.html

Detail API

https://ejpai.journals.ekb.eg/service?article_code=387074

Order

387,074

Type

Original Article

Type Code

643

Publication Type

Journal

Publication Title

The Egyptian Journal of Pediatric Allergy and Immunology

Publication Link

https://ejpai.journals.ekb.eg/

MainTitle

Serum and urinary galactose-deficient IgA1 as a tool for diagnosis of IgA nephropathy in pediatric patients with hematuria

Details

Type

Article

Created At

24 Dec 2024