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294360

IL 13rs20541 Single Nucleotide Polymorphism and Serum IL -13 Level in Children with Bronchial Asthma

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Last updated: 24 Dec 2024

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Abstract

 Background: Bronchial asthma is a common respiratory illness affecting adult and pediatric populations. Interleukin (IL)-13 is a central key mediator of allergic inflammation, and it plays a pivotal role in the pathogenesis of asthma. Several studies revealed that genetic variants of IL-13 promoter rs1800925 allele play a role in asthma severity. We sought to investigate the role of IL-13 rs20541 single nucleotide polymorphism (SNP) and serum IL -13 level in relation to asthma severity in a group of asthmatic children. Methods: This controlled cross-sectional study included 80 participants: 40 apparently healthy controls and 40 asthmatic children, subdivided into three groups: mild (n=12), moderate (n=18 cases), and severe (n=10), recruited from Pulmonology Unit, Children's Hospital, Ain shams university. Characterization of IL-13 rs20541 SNP was achieved by TaqMan real-time PCR. Serum IL-13 concentration was measured using a commercial sandwich ELISA assay. Results: The dominant genotype as regards IL-13 rs20541 SNP among patients and controls was GA (60% and 92.5%, respectively). G and A allele frequencies between patients and controls differed significantly (p =0.002) as the dominant allele in the control group was G allele (70.0%), versus the A allele in the patients' group (53.8%). Patients had statistically significantly higher levels of IL-13 compared to controls (median= 45 pg/ml versus 4 pg/ml; p<0.001) Serum IL-13 correlated positively with IgE levels and was able to discriminate between patients with severe asthma and those with mild to moderate asthma at cut off value of >83 pg/ml (sensitivity 90%, specificity 90%, positive predictive value 96.4% and negative predictive value 75%). Conclusion: IL-13 rs20541 SNP polymorphism is associated with asthma in Egyptian children, while serum IL-13 is linked to the severity of asthma

DOI

10.21608/ejpa.2023.170190.1045

Keywords

Alleles, allergy, genotypes, SNP, cytokine

Authors

First Name

Mona

Last Name

Khattab

MiddleName

Adel

Affiliation

Medical Microbiology &amp; Immunology department, Faculty of Medicine Ain Shams University

Email

monaadelkhattab@yahoo.com

City

Egypt

Orcid

0000-0002-0689-2543

First Name

Mahitab

Last Name

Hussein

MiddleName

Morsy

Affiliation

Department of pediatrics, faculty of medicine, Ain shams university, Cairo, Egypt

Email

mahitab.hussein@med.asu.edu.eg

City

-

Orcid

-

First Name

Walaa

Last Name

Khater

MiddleName

Shawky

Affiliation

Medical microbiology and immunology department, faculty of medicine, Ain shams university

Email

walaa_khater@med.asu.edu.eg

City

-

Orcid

-

Volume

21

Article Issue

1

Related Issue

40729

Issue Date

2023-04-01

Receive Date

2022-10-24

Publish Date

2023-04-01

Page Start

27

Page End

33

Print ISSN

1687-1642

Online ISSN

2314-8934

Article File

EJPA_Volume 21_Issue 1_Pages 27-33.pdf

PDF . 307.0kB

Link

https://ejpai.journals.ekb.eg/article_294360.html

Detail API

https://ejpai.journals.ekb.eg/service?article_code=294360

Order

294,360

Type

Original Article

Type Code

643

Publication Type

Journal

Publication Title

The Egyptian Journal of Pediatric Allergy and Immunology

Publication Link

https://ejpai.journals.ekb.eg/

MainTitle

IL 13rs20541 Single Nucleotide Polymorphism and Serum IL -13 Level in Children with Bronchial Asthma

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Article

Created At

24 Dec 2024