Background: Agile leadership and workplace spirituality are interconnected, as agile leadership fosters an environment of trust, collaboration, and continuous improvement, which aligns with the values of workplace spirituality. This synergy enhances employees' sense of purpose and belonging, leading to greater job satisfaction and engagement. Aim of the study: the current study aimed to assess agile leadership behaviors and its influence on workplace spirituality among staff nurses. Design: a descriptive correlational design was used. Setting: the study was conducted at Ain Shams University Hospital which affiliated to Ain Shams University Hospitals. Subjects: the study subjects include (186) out of (350) staff nurses. Tools: two tools were used namely agile leadership behaviors and workplace spirituality questionnaire. Results:  half (50%) of staff nurses had a low perception level regarding their total agile leadership, while more than half (56.5%) of staff had low perception level regarding their total workplace spirituality. Conclusion: there was highly significant statistically positive correlation between agile leadership behaviors and workplace spirituality among staff nurses. Recommendations: supporting organizational culture that contribute to the growth of the concept and strategies of agile leadership and its effect on the workplace spirituality, Implement agile leadership training for head nurses to improve staff nurse's workplace spirituality.