Background: The decline of biodiversity, growing social inequality, and the irresponsible use of limited resources are putting pressure on organizations to adopt new, sustainable practices. But there is a gap and insufficient attention in the evidence on hospitality for the significance of workplace spirituality and how it helps encourage employees to behave sustainably. The research aimed to investigate organizational sustainability and its relation to workplace spirituality and self-esteem among nursing staff. Research design: research design was a descriptive correlation. Setting: The study was applied on two hospitals at the Minia governorate. Subjects: convenience sample (no.= 277). Data collection Three tools were used, 1^st tool was organizational sustainability, 2^nd tool was workplace spirituality, and the 3^rd tool was self-esteem. Results: clarifies that (65.7%) of nursing staff have high level responses for total organizational sustainability, and (56.0%) of them have high level responses for total workplace spirituality, in addition (75.5%) of them have normal self-esteem. Conclusion: There was a strongly positive correlation between organizational sustainability and workplace spirituality as well as self-esteem among nursing staff.. Recommendation: Conduct a training program by Hospital administration for various levels of nurse managers to enhance work spirituality.