Background: Aluminium phosphate (AP) can be fatal if used for suicidal purposes. The phosphine gas, which is released as a result of aluminium phosphate intake, has highly level of toxic. It causes hypoxia at the cellular level and disrupts oxidative phosphorylation. Intoxication from aluminium phosphate can result in acid-base disorder, heart rhythm abnormalities, acute respiratory distress, and circulatory collapse. Ingesting large amounts can lead to death within hours. Purpose: To assess the effect of educational program on role of nurses in first aid for patients having toxic aluminium phosphate suicide. Design: This study utilized a quasi-experimental research design. Setting: the study was performed at the general and university hospitals in Beni-Suef University. Subjects: The study included 100 emergency nurses. Instruments: Three instruments were employed: (Nurses ‘knowledge assessment Interview Questionnaire, a checklist for evaluating nurses' practices and nurses' attitudes checklist). Results: Illuminate that majority of study group (85%, 82%) and over half of them (55%) respectively had poor knowledge, practice, and attitude levels Pre-program, while almost three-fifths (54%, 64%) and almost of study group(86%) had good knowledge, skill, and attitude level following the implementation of educational program (P> 0.01*).There were a statistically marked difference in the overall scores for knowledge, practice, and attitude levels pre and post-test after application nursing educational program (P> 0.01*). Conclusion: Nursing educational program had favorable effect in improving nurses' knowledge, practice, and attitude regarding first aid emergency response to toxic aluminium phosphate tablet related suicide cases. Recommendations: Replicating this study with a larger sample of nurses across various hospitals is required.