Background: Spiritual leadership styles tend to find employees' have a high satisfaction. Aim: This study was aimed to assess the relation between spiritual leadership and job satisfaction among head nurses. Subjects and Methods: The subjects include (60) head nurses and their assistants. Research design: Cross –sectional analytical design was used. Setting: This study was conducted at all critical and noncritical departments units in Aswan University Hospital. Results: 66.7% of the head nurses and their assistants had high spiritual leadership, 68.3% of the head nurses had high job satisfaction, significant correlation (p<0.01) between spiritual leadership domains and total job satisfaction scores. Conclusion: The result Shows that statistically significant relation (<0.001) between total spiritual leadership and total job satisfaction. Recommendations: Hospital administration should review the system of incentives and seeking alternative methods for recognition of good work, Develop innovative and creative approaches of leadership, decision making, and problem solving to meet ongoing challenges in nursing profession. Implications of spiritual leadership and employ its theory in the future research and its effect on organizational performance and employee effectiveness.