Background: Surgical Site Infection (SSI) is associated with increased morbidity, increased patient's hospital length of stay, re-admission, and excess utilization of health care resources. The bundle approach ensures consistent implementation of all measures to reduce SSI. Aim of the study: was to assess nurse's performance regarding bundle of care for prevention of wound site infection. Design: A descriptive exploratory design was utilized in carrying out this study. Settings: The study was conducted at the general surgical units and the operating rooms at Bahtim Hospital affiliated to Ministry of Health. Study subjects: A convenient sample of all available nurses (40) (male and female) and purposive sample of all available patients during six months of the study duration. Data collection tools: 1. Nurse's self-Administered Questionnaire. 2. Nurse's practice observational Checklists and 3. Patients' clinical data check list. Results: It was revealed that, 67.5% of the studied nurses had unsatisfactory level of total knowledge regarding bundle of car for prevention of SSI. While 77.5% of them had unsatisfactory level of total practice regarding to bundle of car for prevention of SSI. Conclusion: It was concluded that there was a statistically significant correlation between the studied nurses' total level of knowledge and their total level of practices regarding to bundle of car for prevention of SSI Recommendations: Health education program regarding application of bundle of care for prevention of SSI, should be incorporated into comprehensive surgical nursing quality improvement programs to improve nurses' performance and maintain patient safety.