Background: Occupational Health has significantly evolved, becoming a crucial component of national economies, with occupational health and safety units operating inside the personal health sector, fundamental to global civilization. Aim: Assess the occupational health hazards and safety measures among workers at Egyptian Chemical and fertilizers Industries Company at Aswan City, Upper Egypt. Design: A cross sectional design was utilized in this study. Setting: The study was performed at Egyptian Chemical and fertilizers Industries Company at Aswan City, Upper Egypt. Sample: A systematic random sample of 332 workers was employed. Tools: two tools. Tool (I): An interviewing questionnaire sheet comprised three parts: part one: personal data and work data of participants. Part two: past History of occupational hazards. Part three: Worker's knowledge about occupational health hazards. Tool II: Observational checklist to evaluate worker's practice and environmental sanitation. Results: The study revealed that 46.1% of the workers possess (an adequate level of)good knowledge about occupational hazards. Additionally, 76.2% of the workers demonstrated had a satisfactory level of practice concerning the use of Personal Protective Equipment (PPE). Conclusion: There was a statistically significant association correlation (P = 0.043) among the overall level of knowledge and the overall level of practice within the studied workers. Recommendations: Regular periodic screening for all workers for early detection of any health problems.