Background: A surgical site infection (SSI) is the second most common type of healthcare-associated infection. Even though surgical site infection is considered one of the most preventable healthcare-associated infections (HAI), it remains a leading cause of postoperative morbidity and mortality in children who undergo cardiac surgery. Aim: to evaluate the effect of multimedia educational program on the pediatric nurses' performance toward surgical site infection prevention in pediatric intensive care unit. Design: A quasi- experimental design (Pre & Post-Test) was utilized. Setting: The study was conducted at pediatric intensive care unit affiliated with Mansoura University Children's Hospital, Egypt. Sample: A convenient sample of 40 pediatric nurses was included in the study. Tools for data collection; the first consists of two parts: Part I deal with the personal and professional data of the nurses. Part II is based on the pediatric nurses' knowledge regarding surgical site infection. The second is an observation checklist to assess nurses' practice toward surgical site infection prevention. Results: most of the studied nurses after the multimedia education implementation had satisfactory knowledge and competent practice level on surgical site infection prevention with highly significant differences. Conclusion: the study concluded that the multimedia education program regarding surgical site infection prevention had a positive effect on the nurses' performance. Recommendations: Further research should also assess the best teaching strategies, other than the multimedia approach, to improve nurses' SSI prevention performance.