Background/Objective: In the opinion of the World Health Organization [WHO], society's true assets are healthy mothers and children. First, a woman's life will change significantly during her pregnancy, making it a difficult period. Having a child means undergoing a significant self-reconstruction. This study aimed to assess pregnant mothers' knowledge about prenatal care, and Barriers Women Face to attending antenatal care units. Methods: This is a descriptive cross-sectional Community-based study, which was conducted in the El-Suki Locality (Tirara village), Sennar state, Sudan among seventy-two (72) pregnant mothers who were selected by using simple random sampling. Data was collected using a face-to-face self-administered questionnaire. Results: This study's findings indicated that the knowledge level concerning prenatal care among the vast majority of participating mothers was Fair knowledge (54.2%) with all items included in this study. In comparison, few of the study group (12.5%) had good knowledge regarding Prenatal care. The study reported a highly significant correlation (p-value =0.00). between mothers' level of education with their level of knowledge and two-thirds of participants said the main barrier faced them to reach the prenatal care units was the distance between centers and their homes. Conclusion: The study concludes that participants have a fair knowledge regarding prenatal care, and the majority of participants explained that the obstacles and barriers they face in not going to pregnancy care centers are that, centers are so far from their homes, so the researcher recommended that prenatal care centers should be available in all villages and health education programs should be established.