Background: Percutaneous laparoscopic gastrostomy is a way to provide enteral access in patients unable to maintain adequate nutrition via the oral route. Educational Guidelines is the key to positive outcomes in patient requiring enteral feeding. Aim: This study aimed to evaluate the effect of educational guidelines on nurse performance for patients undergoing percutaneous laparoscopic gastrostomy. Design: A quasi-experimental design with pre/posttest was utilized to meet the aim of the study. Setting: This study was conducted at Gastroenterology Endoscopy unit in Medicine Hospital affiliated to Ain Shams University Hospital, Cairo. Egypt. Study subject: A convenience sample of all available nurses (30) caring for patients undergoing percutaneous laparoscopic gastrostomy in the previously mentioned unit. Tools: Ι. Nurses' self-administered questionnaire, ΙΙ. Nurses' practice observational checklists. Results: The study findings revealed that 66.67% of studied nurses had satisfactory knowledge, and 33.33% of studied nurses had competent practices regarding care of patients undergoing percutaneous laparoscopic gastrostomy post implementation of educational guidelines, with statistically correlation between satisfactory and unsatisfactory knowledge and competent and incompetent practice pre and post implementation of the educational guidelines at p= 0.001. Conclusion: Educational guidelines improved nurse's performance regarding care of patients undergoing percutaneous laparoscopic gastrostomy. Recommendations: An orientation program should be prepared to help the newly hired nurses to revise, acquire and develop the knowledge and practice regarding care of patients undergoing percutaneous laparoscopic gastrostomy.