Lean management, rooted in the Toyota Production System (TPS), takes a holistic approach. It emphasizes innovation, efficiency, and customer value while prioritizing quality, safety, satisfaction, and morale within health organizations. Rather than solely cost-cutting, Lean aims for sustainable improvements. This research aimed to Investigate the effect of lean training program on head nurses' knowledge, readiness for lean transformation, and lean culture in Kafr EL- Dawar General Hospital. Methods: Quasi-experimental design was used in 31 units affiliated with Kafr EL- Dawar General Hospital. two groups of subjects participated in the study: all head nurses (HNs) (N= 60), and 250 convenient sample from different nursing qualifications (e.g. diploma, technical, baccalaureate) who are working in the 31 units affiliated with Kafr EL- Dawar General Hospital. Data had collected using four tools: Lean Knowledge Questionnaire (LKQ), Individual Readiness for Lean Transformation Questionnaire, Lean Culture Dimension Framework (LCDF), and Training Program Evaluation Questionnaire (TPEQ). Results: statistically significant differences had found in HNs overall lean knowledge level and readiness level for lean transformation immediately post and after three months of the training program implementation (p<0.05). Also, statistically significant differences had found in nurses' perception of overall lean culture after three months of the lean training program implementation (p<0.05). HNs recorded the lowest percentage of readiness for lean transformation at the principal support dimension. Conclusion: The Lean Training Program significantly improved knowledge level, readiness level for lean transformation among HNs. As well as Nurses' perception of lean culture in Kafr El Dawar General Hospital. The findings highlighted the effectiveness of the Lean Training Program in enhancing lean practices and fostering a culture of continuous improvement within healthcare organizations. Recommendation: future longitudinal studies will shed more light on the causal relationship between Lean knowledge, readiness, and lean culture in healthcare.