Background: The quality of care nurses provide can be significantly impacted by occupational stress, which can also negatively affect their quality of life. Tai Chi has three key components, including meditation, movement, and breathing. The set of relaxing, rhythmic, and fluid movements brings about a state of mental health and calm by fostering the body and mind as a coherent unit as well as evacuating the mind from thoughts that cause tension. Hence, the current study aimed to evaluate the effectiveness of Tai Chi exercises on stress among pediatric nurses at the pediatric intensive care unit. Research design: A quasi-experimental research design was used to fulfill this study using a pre-test and post-test one-group design. Setting: The study was carried out in the neonatal intensive care unit, at Sohag University Hospital, Egypt. Subject: A convenient sample that included (50) nurses was selected. Tools of data collection: Three tools were used; Tool (I): Personal data of the nurses and Tool II: Perceived Stress Scale-10 (PSS-10). Results: A statistically significant difference was found between stress mean scores at (P=0.001) pre and post-Tai Chi exercise intervention. Also, a substantial reduction in pre- and post-Tai Chi exercise intervention between the groups was reported regarding stress levels. Conclusion: The tai chi exercise intervention has resulted in a significant decrease in mean post-test stress scores among pediatric nurses. Recommendations: Provide a training program for pediatric nurses at the pediatric intensive care unit about the importance of the Tai Chi exercise intervention to be able to use them as a part of routine care.