Background: Today's healthcare environment, ambidextrous leadership is essential for fostering innovation by balancing the exploration of new ideas with supporting existing processes and encouraging risk-taking. Aim of the study: The study aimed to investigate ambidextrous leadership and its relation to innovative work behavior among staff nurses. Research Design: A descriptive correlational design was utilized in this study. Settings: This study was conducted at Cardio Vascular Hospital, that is affiliated to Ain Shams University Hospitals. Subjects: The study included 106 of the staff nurses. Tools of data collection: Two tools namely Ambidextrous Leadership Scale and innovative work behavior scale. Results: The overall ambidextrous leadership among staff nurses shows that a notable 83.0% perceive it as high. However, the remaining 12.3% of nurses rate ambidextrous leadership as moderate, and only 4.7% as low. Moreover, 80.2% of staff nurses rate their innovative work behavior as high. Conversely, 15.1% rate their behavior as moderate, and 4.7% as low. Conclusion: A statistically significant positive correlation was found between overall ambidextrous leadership and innovative work behavior among staff nurses. Recommendation: Introduce training programs for nurse leaders to improve their ambidextrous leadership skills by emphasizing the balance between exploring new ideas and optimizing existing processes.