Background: Entrepreneurship is the heart of creativity towards positive change, and it is one of the important sources of economic development and job growth in a country. Growth rate of entrepreneurship varies from country to country and even from time to time in the same country. Aim: Determine the effect of entrepreneurship training program for nursing students, on their entrepreneurial intention and motivation. Design: Quasi-experimental, research design used to conduct this study. Setting: This study was conducted at Faculty of Nursing, Damanhour University. Subjects: A simple random sample was used in this study and included a sample of 4th year male and female nursing students for the academic year 2021-2022 (n=122) who was available and agree to participate in this study Tools: four tools were used for data collection in this study. Tool one: Entrepreneurship Knowledge Assessment Questionnaire. Tool two: Entrepreneurial Intention Questionnaire. Tool three: Entrepreneurial Motivation Questionnaire. Tool four: Training Program Evaluation Questionnaire.  Results: There was a highly positive statistically significant correlation between nursing student's entrepreneurship knowledge and both of entrepreneurial intention and motivation where, p =0.001. and There was positive significant correlation between nursing students' entrepreneurial intention and entrepreneurial motivation. Conclusion:  there was a positive statistical significance correlation between nursing students entrepreneurship knowledge and entrepreneurial intention and motivation and most of 4th year nursing students had a satisfactory knowledge about entrepreneurship during immediately post and follow up (after 1 month) program phase compared with pre-program phase and more than half of studied nursing students had low level regarding overall entrepreneurial intention at preprogram phase, but it is improved to be high level at immediately post program and follow up, near half of studied nursing students had moderate level regarding overall entrepreneurial motivation at preprogram phase, but it is improved to be high level at immediately post program and follow up. Recommendations: Adding a course about entrepreneurship and courses that serve entrepreneurship to educational programs from undergraduate to doctoral program at different faculties of nursing as financial management courses, marketing courses and business planning courses which will help entrepreneurs in business development.