Background: Prediabetes has been considered to be a reversible condition; a modification of lifestyle and other interventions can be successfully applied during the prediabetes period to prevent the development of type 2 diabetes. Aim of the study: To assess the risk factor of prediabetes among female health college students at Najran University. A cross-sectional study was conducted in three health colleges at Najran University, including medicine, nursing, and applied college. A total of 402 study participants of female students were eligible to participate in the study, which was conducted from January to February 2018. Data were collected through an electronic questionnaire. Part I: Socio-demographic data. Part two II: Risk factors for prediabetes. Part III: Knowledge assessment tool, Part IV: Attitude towards insulin resistance& Part V: Practice to ward IR. Results: More than half (64.9%) of females had good knowledge of prediabetes levels. Moreover, there was a statistical association between demographical characteristics according to smoking status and knowledge of risk factors for prediabetes among female health college students. The results of the present study revealed that 64.9 % of female students had a good level of knowledge about prediabetes &92.3%of them had a positive attitude toward prediabetes, and 87.8% had a good level of practice toward prediabetes; additionally, statistically significant positive correlations were found between firstly; students' knowledge and attitude (r =.159, P = .001*). Secondly, between students' knowledge and practice (r = 242, P= .000**). Recommendation: Educational curriculum and facilities should be applied to enhance knowledge according to risk factors for prediabetes. Appropriate and stringent associated factors in lifestyle modifications need to be implemented to minimize the risk of developing prediabetes later in life.