Background: Infertility is a source of social and psychological suffering for around one in six couples. Over the past few decades, remarkable progress has been made in modern reproductive technology to solve this problem. For increasing success rate, educational guideline is essential for improving nurses' performance. The aim of this study was to evaluate effect of educational guideline on nurses' performance regarding assisted reproductive technology. Research Design: A quasi experimental study was used to conduct this study. Setting: The study was conducted in IVF unit at Ain Shams University Maternity Hospital. Sample: Purposive sample consisted of 130 nurses working in the previously mentioned setting and meet the sample criteria. Tools: two tools were used. 1: structured interviewing questionnaire was used to assess general characteristics, nurses' knowledge regarding infertility, infertility treatments, assisted reproductive technologies, artificial insemination and in-vitro-fertilization. 2: practice observational checklists to assess nurses' practice regarding artificial insemination and in vitro- fertilization. Results the current study revealed that the majority of studied nurses have good level of total knowledge about in vitro fertilization and artificial insemination post implementation and three months after implementation of educational guideline, while the majority of the studied nurses were competent regarding in vitro fertilization and artificial insemination post implementation of educational guideline and after three months. Conclusion: The present study concluded that the majority of the studied nurses were competent and had a good level of total knowledge about in vitro fertilization and artificial insemination after the implementation of educational guidelines. Moreover, there was a positive correlation with high statistical significance between the total score of knowledge and the total score of practice regarding artificial insemination and in vitro fertilization. Recommendation: In light of the findings of this study, the researcher recommended that application of educational guidelines for nurses working at IVF units who offer care for women seeking for assisted reproductive technologies and conducting educational training programs about role of nurse regarding assisted reproductive technologies (IVF & AI).