Background: The COVID-19 pandemic has caused not only the risk of death from the viral infection but also unbearable psychological stress to people throughout the world. Aim To assess psychological stressors facing employees working at El Fayoum University hospital during COVID-19 pandemic. Design: descriptive explanatory design was utilized. Setting: The study was conducted at El Fayoum University hospital. Sample: convenient sample of 300 employees working at El Fayoum university hospital. Tool: Two tools were used to collect study data; 1) Interview questionnaire to assess Socio-demographic data of employees working at El Fayoum university hospital, 2) The employees psychological Stress questionnaire to assess psychological stressors facing employees working at El Fayoum university hospital. Result: This study showed that more than one third of the employees working at El Fayoum university hospital were between the ages of 30 to less than 40 years. Meanwhile, more than two thirds of them are females and revealed that more than half of employees working at El Fayoum university hospital have high psychological stresses related to the covid-19 pandemic, on the other hand almost half of them have high social stresses related to the covid-19 pandemic and nearly half of them have moderate physical stresses related to the covid-19 pandemic. Conclusion: More than half of employees facing high level of psychological stressors during covid-19 pandemic, nearly half of them have high level of social stressors during covid-19 pandemic and less than half of them facing moderate level of physical stressors during covid-19 pandemic. Recommendation: Psycho-educational programs for employees about how to face their psychological stresses during covid-19 pandemic, that could curtail the negative impacts of this crisis and eliminates psychological stress of health care employees.