Aim: This study aimed to assess the knowledge, attitude, and practice of the Najran population about blood donation in Saudi Arabia.Methods: A descriptive study was carried out in Najran city to assess people's knowledge, attitude, and practice about donating blood. The study enrolled three hundred and three participants. convenience sampling was used to select participants. Data was collected by questionnaires and analyzed by SPSSResults: There were 303 participants, the majority of whom were female, and 87.5 percent of them had intermediate knowledge regarding blood donation. The majority of participants strongly agreed that they have a good attitude regarding blood donation when asked about it. Additionally, there was no statistical correlation between the research sample's demographic characteristics such as age, social status, education nationality, and knowledge of blood donation, but there was a statistical correlation between these characteristics and their attitude toward blood donation.  Conclusion Indicates that the majority of participants had intermediate knowledge levels, and a favorable attitude toward blood donation and 40% of them had experienced an episode of anemia in the past. In addition, 23% of people simply stated that they had donated in the past. Recommendations: The findings showed the critical need for efficient health education that aimed to inform individuals about blood donation and its effects. There should be more research done on a broader population using more samples.