Background: Negative pressure wound therapy is a highly effective treatment method that is well acknowledged for its diverse benefits in accelerating the healing of wounds. Smooth incorporation of nursing guidelines for patients with negative pressure wound therapy plays a crucial role in reducing problems and maximizing therapeutic results on wound status outcomes. Aim: This study aimed to evaluate effect of nursing intervention guidelines on wound status outcomes for patients undergoing negative pressure wound Therapy. Materials and Methods: A quasi-experimental design was used. A purposive sample of 50 adult patients undergoing negative pressure wound Therapy diagnosed with chronic wounds in the inpatient surgery department at Suez Canal University Hospital. Data were collected using the Patients' Profile questionnaire, and Bates-Jensen Wound Assessment questionnaire. Results: The mean age of patients was 54.60±13.49. There was a statistically significant improvement in total wound status score in the post- and follow-up interventions compared with pre-intervention, with mean scores of 30.34 and 18.78 in both post- and follow-up compared with a mean value of 46.32 in preintervention with a  P value <0.001* in a patient with negative pressure wound therapy. Also, there was a significant improvement across all wound status outcome parameters.  Moreover, parameters like size and epithelialization demonstrated the most considerable changes with a substantial mean reduction from 3.86 and 4.30 (pre-intervention) respectively to 1.40, and 1.82 (follow-up), accompanied by substantial effect sizes ranging from1.4 to 2.9 and 2.0 to 3.3.respectively. Conclusion: There was a significant improvement in the total wound status outcome score in the post and follow-up interventions compared with pre-interventions in patients with negative wound therapy. Educational guidelines were effective for the improvement of wound status outcomes. Recommendations: Nursing intervention guidelines for patients with negative pressure wound therapy should be implemented regularly as a key to improving wound status outcomes..