Background: Sleep disturbances are more common and have a greater impact on morbidity and death in people with chronic illnesses. This study aimed to evaluate the effect of instructional guidelines on sleep quality and functional status in elderly people with chronic kidney disease. Method: Design: A quasi-experimental research design was used to accomplish this study. Setting: The study was carried out at the geriatric home in Mansoura governorate, Egypt. Subjects: A convenient sampling technique of 100 patients who were undergoing hemodialysis within six months, admitted to the previously mentioned setting. Tools: Data was collected by using three tools; demographic and clinical data structured interview schedule, Functional Independence Measure, and Pittsburgh Sleep Quality Index. Results: There were statistically significant improvements in sleep quality and functional status among chronic kidney disease elderly patients pre & post-training instructional guidelines (P<0.001). Conclusion: The present study concluded that the instructional guidelines had a positive effect on improving sleep quality and functional status among chronic kidney disease elderly patients. Recommendations: There is a need for developing comprehensive simple Arabic printed educational materials such as (booklets, pamphlets, and posters) for chronic kidney disease that can improve functional status and quality of sleep. Replication of the current study with a larger sample of patients in different settings is required to generalize the results.