Background: Nurse entrepreneurs play a vital role in the healthcare system by promoting the development of specialized services, such as nursing entrepreneurship and nursing businesses. The research aimed to examine the relationship between Entrepreneurship Head Nurses' Characteristics and their Leadership Practices in University Hospitals. Research design: A descriptive correlational research design was used in this research. Setting: The study was conducted at all Minia University hospitals (university hospital, cardiothoracic, obstetric & pediatric, kidney disease hospital, and liver hospital).  Subjects: The study subjects consisted of all head nurses working in Minia University hospitals, and their total no 85. Data collection: Two tools were utilized Entrepreneurship characteristics Questionnaire and Leadership Practices Inventory. Results: The study's findings demonstrated that more than two-thirds of the studied head nurses have entrepreneurship characteristics. Also, the majority of them have leadership practices. Conclusion: a statistically significant positive association between head nurses' characteristics of entrepreneurship and their leadership practice. Recommendation: Develop an appropriate training program and curriculum to equip nursing students and experienced nurses with the necessary skills and knowledge for a successful career in nursing entrepreneurship.